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Abstract

This investigation examined 100 individuals who had symptoms of acute inflammation affecting the cornea, conjunctiva, and eyelids. Hilla City's private eye clinic welcomed the patients. For 18--24 hours at 37°C, the materials were cultured in both aerobic and anaerobic conditions. Colony morphology, microscopy, and biochemical testing were used to diagnose Staphylococcus aureus infections. The specific positive cards of the Vitek 2 system were used to isolate these isolates. Out of 100 specimens, 65 65%) were found to be associated with different types of microbes, but only 35(35% of the total) tested positive for S. aureus in culture. Out of the 35 S. aureus isolates evaluated for the presence of the fnbpA virulence factor gene, 33 (94.2% of the total) were determined to be positive. This was confirmed by the presence of bands measuring 1362 base pairs when compared to an allelic ladder. Nevertheless, the clfA virulence factor gene was found to be present in 30 (85.7%) of the 35 S. aureus isolates that were analyzed. Supporting this, a comparison with an allelic ladder showed the presence of bands measuring 980 base pairs. Furthermore, the hlg gene was examined in all S. aureus isolates (100%). It was confirmed by comparing the results with an allelic ladder, which revealed bands measuring 937 base pairs. However, out of 35 S. aureus isolates, the pvl gene was discovered in 5(14.2%) of them, with a total of 433 base pairs. The presence of bands measuring 236 base pairs corroborated the discovery of the scpA virulence factor gene in 31(88.5% of the isolates) of S. aureus when compared with an allelic ladder.

Article Type

Original Study

Revise Date

11-12-2024

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